Construction and expression of the eukaryotic coexpression plasmid of Mycobacterium tuberculosis Ag85B and MPT64 gene / 重庆医科大学学报

ABSTRACT

Objective:To construct an eukaryotic coexpression plasmid containing Mycobacterium tuberculosis Ag85B and MPT64 gene.Methods:Mycobacterium tuberculosis Ag85B and MPT64 gene were cloned into pBudCE4.1 to construct recombinant plasmid pBud85B-MPT.The recombinant plasmid was transfected into COS-7 cells,and its expression of Ag85B and MPT64 was assesed by RT-PCR.Results:Mycobacterium tuberculosis Ag85B and MPT64 were detected in transfected COS-7 cells.Conclusion:The recombinant plasmid pBud85B-MPT can express Ag85B and MPT simultaneously in COS-7 cells which provides the basis for further investigation of DNA vaccine against tuberculosis.