Effect of Qinglongyi extracts on cell membrane fluidity of tumor-bearing mice / 中草药

ABSTRACT

Object To study the effects of the different Qinglongyi extracts (QE) on cell membrane fluidity of S 180 and H 22 tumor-bearing mice. Methods The tumor models of S 180 and H 22 were used. The oxter of the normal mice were injected S 180 tumor cell liquid and the abdomen of those were injected H 22 tumor cell liquid. Tumor-bearing mice were given QE by ip cold alcohol extract (191.37, 95.68, 47.84 mg/kg), hot alcohol extract (121.17, 60.58, 30.29 mg/kg), and cytoxan (20 mg/kg as positive control), normal saline (as negative control) for 7 d then the mice were killed, and the tumor cell membrane liquids were made according to the methods. The membrane fluidity was studied with fluorescence polarization (P) and microviscosity (?) with 1,6-dipheyl-1,3,5-hexatriene (DPH) as a probe. Results The QE by both cold and hot alcohol can make the membrane fluidity of S 180 tumor cell improve, the membrane fluidity of H 22 tumor cell decline. Compared with the normal saline control group, QE by both cold and hot alcohol can make the membrane fluidity of RBC (red blood cell) decline (P