Quality standard for Kangmailing Capsule / 中成药

ABSTRACT

AIM: To establish the quality standard for Kangmailing Capsule(Ginkgo Biloba extract, Rhizoma Gastrodiae, Rhizoma Chuanxiong). METHODS: The flavone glycoside content in Kangmailing Capsule was determined by HPLC. Waters ? bondapak C 18(3.9 mm?300 mm, 5 ?m) column was used. The mobile phase was metanol-0.4%H 3PO 4(50∶50), and UV detection wavelength was at 360 nm. Rhizoma Gastrodiae, Rhizoma Chuanxiong, Ginkgo Lactone and Ginkgolic acid in Capsules were identified by TLC. RESULTS: The calibration curves of Quercetin, Kaempferol and Isorhamnetin were linear at the range of 0.150 ?g-1.50 ?g, 0.142 ?g- 1.42 ?g and 0. 096 ?g-0.96 ?g, respectively. The average recovery was all 98.9%, and RSD was 0.98%, 1.15% and 1.57% (n=5). The TLC sports developed were fairly clear, and the blank test showed no interference, the quantitative limitation of Ginkgolic acid was controlled under 5 mg?kg -1. CONCLUSIONS: The established method is accurate,reliable and specific. The results are stable with good reproducibility. The quality of the capsules can be controlled by the method.