Germicidal Actions of the Combination of Herba Houttuyniae and Levofloxacin on Bacterium with Biological Envelope / 中药新药与临床药理

ABSTRACT

Objective To investigate the synergic bactericidal activity of Herba Houttuyniae combined with levofloxacin on the biological envelope of mucoid Pseudomonas aeruginosa(PA).Methods MIC (minimal inhibitory concentration) was determined by using tube doubling dilution method. In-vitro biological envelope models of PA were established by plate cultivation method. The bacterial colony were counted by MTT method. The morphology of biological envelope were observed under scanning electronic microscope (SEM) .Results On the 7th day of cultivation,the stable biological envelope formed.Herba Houttuyniae or levofloxacin alone had no effect on the biological envelope or the number of surviving bacterial colony. The combination of the above two could destroy the structure of biological envelope and decrease the number of surviving bacterial colony.Conclusion Herba Houttuyniae combined with levofloxacin has synergistic germicidal actions on biological envelope of PA.