Effects of component combination in Siwu Decoction on proliferation of human bone marrow stromal cell lines HFCL and hematopoiesis-related gene expression / 中草药

ABSTRACT

Objective To investigate the effects of component combination in Siwu Decoction (CCSWD) on the proliferation of human bone marrow stromal cell lines HFCL and hematopoiesis-related gene expression and to probe into the mechanism of the stimulative effects of SWD and CCSWD on the proliferation of HFCL cells. Methods MTT and FCM were used for assaying the proliferation potency and cell cycle of HFCL cells, respectively. A human hematopoiesis-related cytokine oligonuleotide microarray was prepared to investigate the diversity of gene expression of HFCL cells in SWD and CCSWD groups. Results The energy metabolism and proliferation potency of HFCL cells treated with CCSWD were more prompted than that of the control group. Meanwhile, the percentage of HFCL cells in G 0/G 1 phase treated with CCSWD was significantly lower, while the proliferation index (PI) was higher than that of the control group. Compared to the control group, expressions of IL 2-R?, NF-?B, and TPO were up-regulated in CCSWD group.Conclusion The proliferation of HFCL cells could be facilitated by CCSWD with up-regulating the expressions of IL 2-R?, NF-?B, and TPO.