Preventive and Therapeutic Effect of Kanglaite Injection on Rat Acute Nephrotox icity Induced by Cisplatin and Gemzar / 中药新药与临床药理

ABSTRACT

Objective To observe the preventive and therapeutic effect of Ka ng laite Injection (KI) on acute nephrotoxicity in rats induced by cisplatin (DDP) and gemzar.Methods Forty SD rats, male and female in a half, were randomized in to normal control group, KI group, DDP+ Gemzar group and KI+ DDP+ Gemzar gro up, 5 male and 5 female in each group. The KI+ DDP+ Gemzar group were given K I 10 mL/kg first,DDP injection 6 mg/kg 2 hours later and Gemzar injection 90 mg/ kg 4 hours later. After treatment, acute nephrotoxicity was observed.Results On e week after treatment, all of the rats survived; the body weight of the male an d female rats in group was similar in KI group and in the normal control group, but lower in DDP+ Gemzar group, in particular the male rats, than that in norma l control group. Body weight in KI+ DDP+ Gemzar group was lower than that in the normal control group but higher than that in DDP+ Gemzar group.The serum le vels of biochemical parameters were similar in KI group and the normal control g roup; serum levels of total protein (TP) and albumin(ALB) were markedly lower bu t those of blood urea nitrogen (BUN) and creatine (CRE) higher in DDP+ Gemzar g roup than those in the normal control group; the above indexes were improved in KI+ DDP+ Gemzar group as compared with those in DDP+ Gemzar group and arrive to the normal level.Conclusion KI has preventive and therapeutic effect on acu te nephrotoxicity in rats induced by DDP and Gemzar.