The effect on nitric oxide and nitric oxide synthase of liver injured rat after N-acetylcysteine treatment / 重庆医科大学学报

ABSTRACT

Objective:To evaluate the protective effect of NAC on the acute liver injury of rat and its mechanism.Methods:36 Sprague-Dawley (SD) rats were randomly divided into saline control group (group A,n=12), acute liver injury group (group B,n=12) and NAC intervention group (group C,n=12).In group B&C, SD rats were injected intraperitoneally with D-galactopyranoside(D-gal) (1g/kg). In group C, SD rats were received N-acetylcysteine (NAC) three times after D-gal were given.Animals were killed at 24 hours after the D-gal injection. The pathologic section of rat liver were observed and the concentration of NO and the activity of NOS in rat liver tissue of each group were assayed.Results:The pathologic changes of rat liver were significantly improved in NAC group. The hepatic NO concentration of acute liver injury rats were significantly decreased in the NAC group.Whereas the serum NO concentration was significantly increased in the same group.It showed that the iNOS were inhibited but the cNOS were activated after NAC treatment.Conclusion:NAC can improve the liver tissue pathologic changes of D-gal acute liver injury in rats. The protective activities may associate with the influence on the different types of NOS activities and then change the NO level in different organs in rats.