EFFECTS OF GANODERMA SPORE AND L-NNA ON THE NEURONAL SURVIVAL AND AXONAL REGENERATION IN CLARKE'S NUCLEUS AND RED NUCLEUS AFTER RAT SPINAL CORD INJURY / 解剖学报

ABSTRACT

Objective Whether combination of ganoderma spore and L-NNA(NOS inhibitor) could promote the neuronal survival and axonal regeneration in Clarke's nucleus(CN) and red nucleus(RN) following spinal cord hemisection was explored. Methods Thirty adult female rats were divided into control group,L-NNA group,low dosage of ganoderma spore group,low dosage of ganoderma spore+L-NNA group,high dosage of ganoderma spore group and high dosage of ganoderma spore+L-NNA group.L-NNA was intraperitoneally injected and high dosage of ganoderma spore was irrigated into the rat's stomach after hemisection of T11 spinal cord segment in high dosage of ganoderma spore+L-NNA group. Results Thirty days after spinal cord hemisection,the number of CN neurons was decreased and some neurons were expressing NOS on lesioned side of L1 spinal cord in control group.The number of CN neurons on lesioned side of L1 spinal cord was increased in L-NNA group and low dosage of ganoderma spore group,but the number of NOS-positive neurons was decreased.In low dosage of ganoderma spore+L-NNA group and high dosage of ganoderma spore group,the number of CN neurons was significantly increased and NOS-positive neurons were also significantly decreased on lesioned side of L1 spinal cord.In high dosage of ganoderma spore+L-NNA group,there were most CN neurons surviving and least NOS-positive CN neurons,and some neurons were labeled by flourogold on lesioned side of LI spinal cord.The density of RN neurons on lesioned side was decreased in control group and L-NNA group.The density of RN neurons on lesioned side was increased in low dosage of ganoderma spore group and low dosage of ganoderma spore+L-NNA group.The density of RN neurons on lesioned side was significantly increased in high dosage of ganoderma spore group and high dosage of ganoderma spore+L-NNA group.Conclusion Ganoderma spore or L-NNA can promote the survival of injuried CN neurons after spinal cord hemisection.Combination of ganoderma spore and L-NNA can better promote injuried CN neuronal survival and axonal regeneration.Ganoderma spore can also enhance injuried RN neuronal survival.