NT-3 GENETICALLY MODIFIED SCHWANN CELLS PROMOTE GRAFTED NEURAL STEM CELLS DIFFERENTIATE INTO NEURON-LIKE CELLS IN THE INJURED SPINAL CORD / 解剖学报

ABSTRACT

Objective To explore the effects of Neurotrophin-3(NT-3) genetically modified Schwann cells(SCs) on grafted neural stem cell(NSC) differentiation into the neuron-like cells in the transected spinal cord. Methods The NSCs were single-transplanted or co-engrafted with NT-3 modified SCs,report gene LacZ modified SCs,and unmodified SCs respectively into the transected spinal cord 67d later,the differentiation of NSCs was studied by immunohistochemisty,and the percentage of neuron-like cells was calculated. Results NSCs could differentiate into the neuroglial-like cells and neuron-like cells in the injured spinal cord.Compared to the unmodified SCs,NT-3 modified SCs could more efficiently promote NSCs differentiate into the neuron-like cells.The effect of LacZ modified SCs was the same to the unmodified SCs.Conclusion NT-3 modified SCs could promote NSCs differentiate into the neuron-like cells in the injured spinal cord.