A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism / 重庆医科大学学报

ABSTRACT

Objective: To investigate the apoptosis of isolated pancreatic islet cells and insulin secretion induced by combination of IL-1?,TNF and IFN-?,and to investigate the mechanism of the change.Methods: Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro.NO production,NOS activity,insulin secretion,the protein expression of bcl-xl and bax,apoptotic rate of islet cells and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1?,TNF and IFN-? were measured.Results: Combination of IL-1?,TNF and IFN-? induced a significant increase in apoptotic percentage of pancreatic islet cells,NO production and NOS activity,as well as DNA ladder appearance,while a decrease in insulin content.Up-regulation of the protein expression of bax and down-regulation of the protein expression of bcl-xl were also induced(P