Inhibitory effects of TIMP-3 on endothelial cell proliferation / 重庆医科大学学报

ABSTRACT

Objective: To investigate the inhibitory effect of TIMP-3 on endothelial cell(EC) proliferation.Methods: The inhibition of EC proliferation was analyzed by light microscopy,MTT,-TdR up-take,and flow cytometry et al.Results: A concentration-dependent and time-dependent proliferation inhibition and survival were demonstrated in the HUVEC incubated for 1~7days in the presence of TIMP-3.The proliferation inhibition was shown as the lessened cell growth,decreased counting of mitotic cells.TIMP-3 could also aggravate the increase of apoptotic cells and cell ratio in G2/M phase.Conclusion: TIMP-3 has significant inhibitory influence on EC proliferation,migration and can promote apoptosis of cells.