RAPD Analysis on farm cultivars of fruits of Trichosanthes kirilowii from Shandong Province / 中草药

ABSTRACT

Objective To explore the genetic diversity and relationship of different cultivars of the fruits of Trichosantheis kirilowii, and determine them in molecular level. Methods Different fram cultivars and fruits in wild plant of T. kirilowii from Shandong Province and T. hupehensis were detected by randomly amplified polymorphic DNA (RAPD) markers with 25 randomized primers. The Jaccard coefficient was worked out by using NTSYS-pc software, and a cluster dendrogram of different samples was established based on unweighed pair-group method with arithmetic mean (UPGMA). Results The 25 selected primers produced 266 bands among which 232 bands (87.2%) were polymorphic. In the cluster dendrogram, T. hupehensis was far from the different cultivars and fruits in wild plant of T. kirilowii, but there was closer genetic relationship between Niuxin Gualou and Ren Gualou, Da Gualou, and Baleng Gualou, as well as Xiao Guang-dan Gualou and wild sample. Conclusion RAPD Markers can be used to standardize, arrange, and determine farm cultivars of fruits of T. kirilowii in molecular level, and indicate the genetic relationship and background, then provide important information for seed breeding and resourse protection of T. kirilowii.