Comparison of Xiexintang Dispensing Granules and standard decoction with 3D-HPLC fingerprint chromatogram / 中成药

ABSTRACT

AIM: To study the difference in main chemical composition and content between(Radix et Rhizoma Rhei;Rhizoma Coptidis;Radix Scutellariae) dispensing granules and standard decoction. METHODS: 3D-HPLC was used with MeOH(A)、0.1%H_3PO_4-H_2O(include 0.02 mol/L KH_2PO_4,pH=3)(B) gradient elution,the fingerprint chromatogram of Xiexintang Dispensing Granules and standard decoction were set up. RESULTS: 30 peaks were indicated,the relative retention time and relative peak area were obtained. CONCLUSION: (The) difference between Xiexintang Dispensing Granules and standard decoction is obvious,the contents of the former are higher.