Research on the experiment of eliciting specific antitumor immunity by fusion of dendritic cell with gastric cancer cells in vitro / 重庆医科大学学报

ABSTRACT

Objective: By hybrid technology,to study the biological characteristics and antitumor function of gastric cancer-dendritic fusion cells through the fusion of dendritic cells from cord blood with gastric cancer cells SGC7901,Method: DCs from cord blood were fused with SGC7901 in the presence of 50% polyethylene glycol.The morphology and phenotype of DCs,SGC7901 and DCs/SGC7901 were respectively observed by SP immunocytechemical staining.The ability of DCs/SGC7901 which stimulated proliferation of autologous T lymphocytes was assessed by MTT,and the kill rate of cytotoxic T lymphocytes(CTL) to SGC7901 induced by FCs(fusion cells) was evaluated through cytotoxic test.Result: DCs were successfully fused with SGC7901 in the presence of PEG,FCs expressed CEA and HLA-DR two phenotypes.Moreover,fusion cells had greater ability to stimulate proliferation of T lymphocytes than DCs?DCs+SGC7901,SGC7901,and the kill rate of FCs(21.4%?0.0165%) was greatty high than that of DCs(16.3%?0.0015%),DCs+SGC7901(15.3%?0.0044%),SGC7901(11.2%?0.0156%).Conclusion: DCs from cord blood can be successfully fused with SGC7901 in the presence of PEG,and FCs can effectively stimulate T lymphocytes to elicit antitumor immunity effect.