Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus / 重庆医科大学学报

ABSTRACT

Objective:To dynamically observe changes of IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).Methods:BALB/C mice were intranasally or orally vaccinated and killed on 0、2、4、6、8、10、12、14、16 and 18w of immunization,with 4 in each killing.Sera were gathered from the eyeball to measure IgG, its subclass and IgE by routine ELISA,with PBS as control.Results:In the intranasal group,levels of IgG、IgG2a and IgG2b increased obviously on 2~18w,and reached the highest level on 10、4 and 6w respectively;while levels of IgG1、IgG3 and IgE decreased remarkably on 2~18w,and came to the lowest level on 8、12 and 10~12w respectively.In the oral group,levels of IgG、IgG2a and IgG2b rose remakably on 2~18w,and came to the peak value on 14、8 and 8w respectively;while levels of IgG1、IgG3 and IgE decreased on 2~18w,and reached the lowest level on 8、6 and 16~18w respectively.Conclusion:TH1 response could be induced in mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus in early immunization.