Study on transplantation of mesenchymal stem cells from human fetal livers into rat infarted heart / 重庆医科大学学报

ABSTRACT

Objective:To study the survival and the cardiac differentiation of mesenchymal stem cells from human fetal livers(FMSCs) after being transplanted into infarted rat myocardium. Methods:FMSCs were cultured from human fetal livers, the average age of which was 9 days. The infarction model was built by the ligation of left anterior descending artery and 3.5?106cells were transplanted into infarction area by direct injection one week after ligation. Rats were killed at 1 and 2 weeks after transplantation, and hearts were collected for the examination of the cells' survival and differentiation toward cardiac tissue by fluorescent in situ hybridization(FISH)and immunohistochemistry staining. Results:The FACS results showed that FMSCs expressed antigen CD29,CD44,CD166, SH2(CD105),SH3 and SH4, but did not express CD14,CD34 and CD45.The transplanted cells survived at 7th day after transplanted,but dissappeared at 14th day. No cardiac differentiation was observed during this period. Conclusion:The cells we cultured are testified as FMSCs by their phenotypic expression,and FMSCs could survive for a short period after transplanted into rat heart infarction model,but no cardiac differentiation is induced.