Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)2007.
Article
en Zh
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| ID: wpr-576912
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ABSTRACT
Objective:To dynamically observe changes of subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em).Methods:BALB/C mice were intranasally vacci- nated with the vaccine and killed to get spleen at 0,2,4,6,8,10,12,14,16 and 18w of immunization respectively.Splenocytes were.separated to measure subsets of CD_4~+ and CD_8~+T cells by FACsort,PBS served as control.Results:In the groups of im- munization,CD_4~+ and CD_8~+subsets increased obviously at 2~12w and 2-18w respectively,which reached the highest level at 6 and 10w respectively.Conclusion:The number of CD_4~+ subsets of splenocytes increases remarkably in the early stage (2~6week)by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine.
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Zh
Revista:
Journal of Chongqing Medical University
Año:
2007
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Article