Quality control of Rhizoma Pinelliae by chromatograph-digitized fingerprint spectrum / 中成药

ABSTRACT

AIM: To establish HPLC-digitized fingerprint spectrum (HPLC-DFPS) of Rhizoma Pinelliae so that it will be applied in quality control of Rhizoma Pinelliae. METHODS: A gradient separated method was applied. Column Agilent Zorbax ODS C_ 18 ; Mobile phase acetonitrile-water; Detection-wavelength 210 nm; Flow rate 0.5 mL/min; Column temperature 25℃. RESULTS: Using above optimum chromatograph condition, the HPLC-DFPS and technic parameters of Rhizoma Pinelliae were established. CONCLUSION: Comparison of a group of characteristic peaks suggests that the HPLC-DFPS technology is feasible in quality control of Rhizoma Pinelliae.