Methodology for HPLC fingerprint analysis on fruits of Amomum villosum / 中草药

ABSTRACT

Objective To establish the method of fingerprint analysis on the fruits of Amomum villosum by HPLC and work out the characteristic fingerprint of methanol extracts in the fruits of A.villosum from Yangchun,Guangdong Province as index component which could be used for the accumulation of data to evaluate the inner quality of the fruits of A.villosum.Methods HPLC with Nucleodur C18 Gravity column was used,the methanol-2% acetic acid(gradient elution)as a mobile phase,detection wavelength at 260 nm,column temperature was 30 ℃,and flow rate was 1.0 mL/min.ResultsCommon peaks(20)were separated on HPLC fingerprint in A.villosum.The similarities of 18 batches of samples were higher.Conclusion The method is reliable and accurate,has a better reproducibility,and provides a reference for the quality control to the fruits of A.villosum.