Effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with Echinococcus multilocularis / 重庆医科大学学报

ABSTRACT

Objective:To detect the effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with protoscoleces of Echinococcus multilocularis.Methods:BALB/C mice were immunized with rBCG-Em Ⅱ/3 vaccine by subcutaneous injection or intranasal inoculation.After 8 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection.On the 18th week after infection,mice were killed to separate splenocytes and stimulated with ConA for 16～18h,then apoptosis of splenocytes was detected by Annexin V-FITC staining methods.Results:Apoptotic rate of splenocytes in all groups with or without ConA stimulation was obviously lower than that of PBS group(P