Study on AdCD-UPRT suicide gene mediated on lung cancer A_(549) in vitro / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)2007.
Article
en Zh
| WPRIM
| ID: wpr-577260
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WPRO
ABSTRACT
Objective:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. Methods:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry. Results:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. Conclusion:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.
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Zh
Revista:
Journal of Chongqing Medical University
Año:
2007
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Article