HPLC fingerprint of Lobelia chinesis Lout / 中成药

ABSTRACT

AIM:To establish the chromatographic fingerprints of Lobelia chinesis Lour.by RP-HPLC which may be potentially beneficial to the quality control in production.METHODS:Separation was performed on a Daiso C_ 18 column with methanol-water as mobile phase using gradient elution at flow rate of 1.0 mL/min.The UV detection wavelength was at 261 nm and the analysis time was up to 60 min.Total ten batches of Lobelia chinesis Lout.were studied.RESULTS:11 peaks were identified as the characteristic fingerprints of Lobelict chinesis Lour.under the above chromatographic conditions.CONCLUSION:The RP-HPLC fingerprint method is found to have satisfactory accuracy,stability and reproducibility.A potential method for the quality control of Lobelia,chinesis Lour.was established.