Experimental study on the effect of angelica polysaccharide on inhibition of proliferation and induced differentiation towards erythroid cell of human erythroleukemia cell line(K562) / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of Angelica polysaccharide on inhibition of proliferation and induced erythrocytic differentiation of human erythroleukemia cell line(K562).Methods:Techniques of cell culture were used in this experiment.The effect of APS on proliferation of K562 was examined by MTT、trypan blue staining and flow cytometry.The change of cell form was observed by Wright's stain.The effect of APS induced erythrocytic differentiation of K562 was detected by cytochemistry and spectrophotometric method.Results:APS can significantly inhibit the proliferation of K562 ex vivo,and the inhibitory extent is paralleled to the concentration and the acting time of APS;APS promoted the expression of Hb.Conclusion:APS can significantly inhibit the proliferation and induce the differentiation towards erythroid cell of K562 ex vivo.