Amplification,purification and identification of human K562 cell cDNA library and its transformation into yeast cell / 重庆医科大学学报

ABSTRACT

Objective:To Amplify,purify and identify the cDNA library of human K562 cell in E.coli DH5? and to transform it into yeast cell for subsequent target protein screening.Methods:The cDNA library of human K562 cell was amplified.Then the library plasmids were extracted and transformed into Y187 yeast cell.The results of transformation were verified by PCR and restriction enzyme analysis.Results:The cDNA library of human K562 cell was amplified successfully and the diversities were verified.The cDNA library of human K562 cell was also successfully transformed into Y187 yeast cell.Conclusion:Successfully amplification,purification and identification of the cDNA library of human K562 cell lay the foundation for the subsequent protein screening.