EFFECT OF MONOCHROMATIC LIGHT ON CELL DENSITY AND CELL SIZE OF RETINAL GANGLION CELLS IN CHICKS / 解剖学报

ABSTRACT

Objective To study the effect of monochromatic light on the distribution of retinal ganglion cells in chicks. Methods Sixty One-day-old male broilers were reared under four light treatments,red(660nm),green(560nm),blue(480nm) and white(400-760nm) by using LED(light-emitting diodes) as light sources until the 49th day(n=15).Light intensity was 15 lux at the height of birds' heads and scheduled for 23hours of light and 1hour of darkness during the entire experiment.The retinas were stained by Nissl-staining and DiI-labeling.The retinal area,cell size and density in ganglion cell layer were estimated by image analysis. Results The retinal area and RGCs' number in the blue and green light groups were higher than that in the red and white light groups(10.35% and 17.07%,P