EFFECT OF CGRP AND NGF ON THE PHOSPHORYLATION OF CREB PROTEIN IN RAT HIPPOCAMPUS DURING FOCAL CEREBRAL ISCHEMIA/REPERFUSION / 解剖学报

ABSTRACT

Objective To investigate the effect of exogenous calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of cyclic AMP response element binding protein(CREB) and phosphorylated CREB(p-CREB) in rat hippocampus during focal cerebral ischemia/reperfusion(I/R). Methods Focal cerebral ischemia/reperfusion model was set up by occlusion of the right middle cerebral artery using the intraluminal suture method.The expression of CREB and p-CREB in the right hippocampus were detected with immunohistochemistrical SABC method and Western blotting.The immunoreactive positive products were analyzed by image analysis system. Results The expression of CREB decreased in I/R group as compared with sham group in right hippocampus,but the expression of p-CREB was higher in I/R group than that in sham group(P