Effects of angiogenin on apoptosis cells in the brain of rats with reversible-ischemic injury / 重庆医科大学学报

ABSTRACT

Objective:The purpose is to observe the change of the number of apoptosis cell in the reversible-ischemia rat brain after the intraperitoneal injection of rhANG. Methods:With the use of a reversible(middle cerebral artery occlusion (MCAO))m odel in rats,apoptosis cells were assessed by(terminal deoxynucleotedyl transferase(TdT))-mediated dUTP-biotin nick end labe ling(TUNEL) histochemical methods in sham,brain ischemia control and rhANG intraperitoneal injection groups.Results:There was no significant difference in the number of the apoptosis cell in the penumbra zone in the ischemic brain between the rhANG intraperitoneal injection group and the brain ischemia control group.The number of apoptosis cell in the ischemic lateral brain was increased significantly compared with the non-ischemia lateral in the brain ischemia control group and the rhANG intraperitoneal injection group. Conclusion:There was no significant difference in the number of the apoptosis cell in the penu mbra zone in the ischemic rat brain after the intraperitoneal injection of rhANG(15ng/100g)compared with the control group.