Determination of Borneol and Menthol in Compound Shexiang Injection by HS-GC / 中药新药与临床药理

ABSTRACT

Objective To establish the determination of borneol and menthol in C ompound Shexiang Injection by HS-GC.Methods A Rtx-Wax column (30 m?0.25 mm? 0.25 ?m) and FID were used.The injector and the detector temperature was 260 ℃ .Column temperature was controlled by programminginitial temperature at 140 ℃and holding 2 min,and temperature increase at 10 ℃?min-1,final temperatur e at 220 ℃for 4 min. Results The linear range of borneol and menthol was 13.025 ～416.8 ?g?mL-1 (r=0.999 5,n=6) and 13.125～420 ?g?mL-1 (r=0.999 7,n=6) ,and the average recovery was 98.97 %(RSD=3.0 %,n=6) and 97.65 %(RSD=2.1 % ,n=6),respectively.Conclusion The method is sensitive,accurate and reproduci ble for simultaneous determination of borneol and menthol in Compound Shexiang I njection.