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Determination of bulleyacinitine A in mouse plasma by liquid chromatography tandem mass spectrometry / 中成药
Chinese Traditional Patent Medicine ; (12)1992.
Artículo en Chino | WPRIM | ID: wpr-578758
ABSTRACT

AIM:

To develop the method for analyzing bulleyacinitine A in plasm from mice through the treatment of subcutaneous injection of bulleyacinitine A by liquid chromatography tandem mass spectrometry(LC-MS/MS).

METHODS:

50 ?L mouse plasma sample added zolpidem as internal standard and pH 9.2 buffer solution was extracted with ethylether,followed by liquid chromatography and mass spectrometric detection.The mobile phase was consisted of methanol-buffer solution(85∶15)(buffer solution consisted of 20 mmol/L ammonium acetate and 0.1% formic solution).The flow rate was 300 ?L per minute.The separation column was C_ 18 column.The electrospray ionization tandem mass spectrometry and the multiple reaction monitoring mode were applied to detecting the bulleyacinitine A in plasma.

RESULTS:

The assay was linear from 0.1 to 1 000 ng/mL.The recovery rate was more than 80%.

CONCLUSION:

The method could be used to detect bulleyacinitine A level in mouse plasma,which offers advantages of sensitiveness,specificity and simpleness.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional Patent Medicine Año: 1992 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional Patent Medicine Año: 1992 Tipo del documento: Artículo