Effects of ?-asarone on Mitochondrial Membrane Potential of Cardiac Myocytes with Ischemia Reperfusion Injury / 中药新药与临床药理

ABSTRACT

Objective To study the effects of ?-asarone on mitochondrial membrane potential(MMP) of cardiac myocytes with ischemia reperfusion injury. Methods The cardiac myocytes model of myocardial ischemia reperfusion injury (MIRI) was induced with sodium dithionite (Na2S2O4). The primary cultured cardiac myocytes of SD neonatal rats were randomized into six groupsnormal group,model control group,high-dose ?-asarone (25 ?g/mL),middle-dose ?-asarone group(12.5 ?g/mL),low-dose ?-asarone group (6.25 ?g/mL) and medium group. Mitochondrial membrane potential(MMP) of myocardial cells were measured by flow cytometry. Results Compared with the normal group,MMP in the model control group was obviously decreased(P