Comparative analysis of ?-tubulin,?-tubulin and ?-tubulin of Immortalized cells and carcinoma cells of the Cervix / 重庆医科大学学报

ABSTRACT

Objective:To investigate the expressions of ?-tubulin,?-tubulin and ?-tubulin of centrosome in immortalized hu-man squamous epithelial cel(lH8)and cervical cancer cel(lCaski)and the role of them in occurrence and development of cer-vical cancer.Methods:Immunocytochemistry was used to analyze the protein expressions of ?-tubulin and ?-tubulin in im-mortalized human cervical cells and carcinoma cells.The skeleton protein was extracted by ProteoExtract Subcellular Proteome Extraction Kit,and then Western blotting was used to determine the protein expressions of ?-tubulin,?-tubulin and ?-tubulin semi-quantitatively.Results:The protein expressions of ?–tubulin,?-tubulin and ?-tubulin in Caski were higher than those in H8(P