Effect of valsartan on heart function and activity of myocardial sarc-oplasmic reticulum Ca~(2+)-ATPase in junior rat with heart failure / 重庆医科大学学报

ABSTRACT

Objective:To explore the effect of Valsartan on heart function and a-ctivity of myocardial sarcoplasmic reticulum Ca2+-ATPase in junior rat with heart failure(HF).Methods:The animal model of congestive heart failure was established byfis-tulation of abdominal aorta and inferior vena cava.Rats were randomly divided into 2 groups after 8 weeks of operationrats without treatment as control group(n=20),rats treated with Valsartan as valsartan group(n=15).Sham-operated was taken as normal control group(n=15).Valsartan was adminis-tered through direct gastric gavage.The high frequency ultrasound was per-formed 4 weeks after treatment.Myocardial SR was fractionated with velocity centrifugation.Enzyme activity of SERCA2a in SR was detected with ultraviolet spectrophotometre(U V).The time course of Ca2+ uptake was determined by fluorescent spectropho-tometry.Results:Compared with the sham-operated group,left ventricular internal dimension diastole(LVIDd),left ventricularin-ternal dimension systole(LVIDs),left ventricular end-diastolic volume(LVEDV),and left ventricular end-systolic volume(LVESV)were all significantly increased(P