Synergistic Action of Radix Angelicae Sinensis and Flos Carthami on Clopidogrel Bisulfate Counteracting Platelet Aggregation / 中药新药与临床药理

ABSTRACT

Objective To study the synergistic action of Radix Angelicae Sinensis(RAS) and Flos Carthami(FC) on Clopidogrel Bisulfate(CB) counteracting platelet aggregation. Methods Forty-eight Sprague-Dawley(SD) rats were randomized into 6 groupsnormal control group,CB group(1 mg? kg-1? d-1,qd),RAS group(0.7 g? kg-1? d-1,bid) and FC group(0.7 g? kg-1? d-1,bid),CB+ RAS group(CB 1 mg? kg-1? d-1,qd,and RAS 0.7 g? kg-1? d-1,bid),and CB+ FC group(CB 1 mg? kg-1? d-1,qd,and FC 0.7 g? kg-1? d-1,bid). The rats received gastric infusion of corresponding drugs for 5 continuous days. Blood sample of SD rats were collected by puncture of the abdominal artery for the observation of platelet aggregation in each group.Another fifty-four rats were used for the observation of inhibitory effect on thrombosis in arteriovenous shunt. Sixty NIH mice were applied for the observation of effect on bleeding time,clotting time and parameters of routine blood examination. Results Platelet aggregation and thombosis in arteriovenous shunt of rats were inhibited,and the bleeding time was prolonged in the medication group. The above effect was much better in the combination groups. The effect on clotting time and parameters of routine blood examination was not obvious. Conclusion Radix Angelicae Sinensis and Flos Carthami can enhance the effect of Clopidogrel Bisulfate on inhibiting platelet aggregation and thrombosis,and on prolonging bleeding time.