Construction and identification of the recombinant pCD-Eg95 plasmid of Echinococcus granulosus / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1987.
Artículo
en Chino
| WPRIM
| ID: wpr-579178
ABSTRACT
Objective:
To construct and identify the recombinant pCD-Eg95 plasmid of Echinococcus granulosus.Methods:
Total RNA was extracted from hydatid cyst protoscoleces shattered by supersound. The Eg95 coding gene was amplified by RT-PCR, and then was cloned into the eukaryotic expression vector pCDNA3.1 to construct the recombinant pCD-Eg95 plasmid. The recombinant plasmid was electroporated into E. coliBL21(DE3) strain.Results:
The 471 bp Eg95 coding gene was successfully amplified, and the recombinant pCD-Eg95 plasmid was successfully constructed, which was confirmed by restriction endonuclease digestion and PCR.Conclusion:
The results could provide the basis for the further research of the nucleic acid vaccine of Echinococcus granulosus.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Journal of Chongqing Medical University
Año:
1987
Tipo del documento:
Artículo
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