Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines / 重庆医科大学学报

ABSTRACT

Objective:To study the effect of RNA interference(RNA)ion ATX-mRNA expression and the invasive petential of human breast cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA)targeting ATX was transfected into human breast cancer cell MCF-7 using SiPORT Lipid.The transfection efficiency was observed under fluorescence confocal microscope.Expression of ATX mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Cell penetrate matrigel capacity were determined by in vitro experiment.Results:ATX-siRNA effectively inhibited ATX-mRNA and protein expression(P