Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection / 重庆医科大学学报

ABSTRACT

Objective:To evaluate the synergism ofrecombinant M.smegmatis(MS)encoding human granulysin and murine IL-12 to anti-tuberculosis chemotherapy in murine M.tuberculosis M.tuberculosis infection.Methods:Balb/c mice were infected with normal saline, recombinant MS,INH(Isoniazid)+PZA(pyrazinamide),recombinant MS+INH+PZA respectively.The numbers of viable bacteria in the lung and the spleen were counted.The levels of IL12 and IFN-?in serum and IFN-?and TNF-?released from spleen lymphocytes stimulated with PPD were detected with ELISA.Lungs and spleens were prepared for pathological analysis.Results:The recombinant MS group,INH+PZA group,and recombinant MS+INH+PZA group showed significantly reduced number of colony forming units(log CFU/g) compared with saline group.The increase of IL-12 and IFN-?in serum were found in recombinant MS group.IFN-?and TNF-?released from spleen lymphocytes stimulated with PPD in recombinant MS group was higher than that in other groups(P