Improvement of primary culture of cardiac myocytes in neonatal rat / 重庆医科大学学报

ABSTRACT

Objective:To discuss how the more convenient primary culture method of rats'cardiac myocytes to increase the quantity,livability and purity of isolated cells.Methods:Neonatal rats'cardiac myocytes were isolated by co-digestion of trypsin and collagenase Ⅱ,and then cultured in vitro.Morphocytology changes were observed and cell purity was evaluated by immunofluorescence.Results:Isolation of cardiomyocytes was with a good quantity and high livability.Cells adhered and impulsed well.Immunofluorescence staining confirmed the cell with purity higher than 95%.Conclusion:The modified cell culture method can obtain the Cardiomyocytes with good growth state.