The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin / 重庆医科大学学报

ABSTRACT

Objective:To investigate the changes of reactive oxygen species(ROS)and Glutathione(GSH) and their chronological order in the apoptosis of K562 cells induced by simvastatin to explore the apoptosis mechanism.Methods:K562 cells cultivated in routine method for 24h were exposed in the simvastatin 20?mol/L.Cell morphological analysis were conducted at 48h and the vitalities of K562 cells were cultivated detected with MTT at different times(12,24,48,72 h) respectively.Fluorochrome flow cytometry was performed to detect cell apoptotic ratio and changes of intracellular ROS.The content of intracellular GSH in K562 cells was measured by colorimetric method.Results:K562 cells presented typical apoptosis morphological changes the treatment of simvastatin for 48h.cell inhibition ratio at different times(12,24,48,and 72 h) were 0,(19.02?0.92)%,(56.4?3.20)%,(74.4?5.54)% respectively and cell growth of K562 was inhibited.The apoptotic ratio were(2.55?0.25)%,(6.1?0.35)%,(14.15?0.42)%,and(30.70?0.65)% respectively.Furthermore,the changes of fluorescence intensity of ROS were also significantly increased(P