Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231 / 重庆医科大学学报

ABSTRACT

Objectives:To investigate the effects of small interference RNA(siRNA) silencing Annexin Ⅱ on invasion and metastasis of human breast cancer cell MDA-MB-231.Methods:The chemically-synthetic siRNA duplexes targeting Annexin Ⅱ(Annexin Ⅱ-siRNA) was transiently transfected into MDA-MB-231 cells which have high metastatic potential by lipofectamin 2000.The transfection efficiency was observed by fluorescence microscopy.RT-PCR and western blot were used to semi-quantify the Annexin Ⅱ mRNA and protein levels.The malignant characters of transfected MDA-MB-231 cells including celluar proliferation rate,the activities of invasion and metastasis were analyzed by use of 3-(4,5-dimethylthiazol-2-yl)-5-diphenyl tetrazolium bromide(MTT) and millicell chamber assay,respectivilly.Results:Annexin Ⅱ-siRNA effectively inhibited Annexin Ⅱ mRNA and protein levels(P