Determination of Phillyin in Siji Ganmao Pian by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish the method for detemining the content of Phillyin in Siji Ganmao Pian by HPLC.Methods Diamonsil ODS1 C18 column was used with acetonitrile-water(25∶75) as the mobile phase,detection wavelength as 277 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.1128～0.9024 ?g for Phillyin and linear equation was Y =5.795 68X +1.843 19,r =0.999 3.The average recovery was 98.6% and RSD was 1.82%(n =5).Conclusion This method was simple,accurate and proper,the reduplication of the result was good.The method can be provide for quantitative analysis of Phillyin in Siji Ganmao Pian.