Curcumin reduces A?generation of neurons by PPAR? activation and BACE1 inhibition / 重庆医科大学学报

ABSTRACT

Objective: To investigate the inhibitory effects of Curcumin on A? generation in vitro and explore its potential mechanisms involing BACE1 and PPAR?.Methods: Plasmids APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells by LipofectaminTM2000,then treated with Curcumin at 0(control Group),1.25,5.0,20.0 ?mol/L for 24 hr,or with Curcumin at 5 ?mol/L for 0,12,24 and 48 hrs for the time course assay.RT-PCR was performed to measure the endogenous levels of BACE1 mRNA and PPAR?.Western blot were used to detect the protein expression of BACE1 and PPAR?.The major cleavage product——A?40 and A?42 was detected by ?-amyloid 1-40 or 42 Colorimetric ELISA assay.Results: The mRNA level and protein level of BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in the cell line(P