Simultaneous determination of notoginsenoside R_1,ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb_1 in Yunnan Baiyao Powder by RP-HPLC / 中成药

ABSTRACT

AIM: To establish a HPLC method for determing notoginsenoside R_1,ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb_1 in Yunnan Baiyao Powder. METHODS: A RP-HPLC method was applied with a LiChrospher C_(18) column(4.6 mm?250 mm,5 ?m) by gradient elution of acetonitrile-0.4% phosphoric acid solution as the mobile phase.The flow rate was 1 mL/min and the detective wavelength was set at 203 nm.The column temperature was at 30 ℃. RESULTS: All of the four compounds showed good linearities and the recoveries were in the range of 99.6%-101.2%. CONCLUSION: The method is accurate and suitable for the quality control of the product.