Induction of dihydroartemisinin on prostate cancer PC-3 apoptosis and its mechanism / 中草药

ABSTRACT

Objective To study the induction of dihydroartemisinin(DHA) on prostate cancer PC-3 apoptosis and its possible mechansim.Methods MTT was employed for cellular viability measurement,flow cytometry(FCM) and transmission electron microscopy(TEM) for observation of apoptosis,and immunocytochemical staining(SP) for analyzing the expression of Bcl-2 and Bax proteins in PC-3 cells treated with DHA of different concentration.Results DHA Significantly inhibited the proliferation of PC-3 cells,induced their apotosis in a time-concentration dependent manner,and led to mitochondrial swelling,nuclear fragmentations and apoptosis body formation,down-expression of Bcl-2 protein,and over-expression of Bax protein correspondence with DHA concentration.Conclusion DHA could induce the apoptosis in PC-3 cells by up-regulating Bax protein and down-regulating Bcl-2 protein.