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Determination of four effective components in Chaihu Shugan Powder by HPLC/DAD / 中成药
Chinese Traditional Patent Medicine ; (12)1992.
Artículo en Chino | WPRIM | ID: wpr-580706
ABSTRACT

AIM:

To establish a method for determining saikosaponin A,paeoniflorin,hesperidin and ferulic acid in Chaihu Shugan Powder(Pericarpium Citri Reticulatae,Radix Bupleuri,Rhizoma Chuanxiong,Fructus Aurantii,Radix Paeoniae alba,etc.).

METHODS:

The chromatographic saparation was performed on a Hypersil C18 column(250 mm?4.6 mm,5 ?m).The mobile phase was acetonitril-water(4357) for saikosaponin A,and the mobile phase for rest components was acetonitrile -0.5% acetic acid(4060).All of flow rates were 0.8 mL/min and column temperature maintained at 30 ℃.The detection wavelength was set at 200-400 nm.

RESULTS:

The four constituents were separated within 15 min.The linear ranges of saikosaponin A,paeoniflorin,hesperidin and ferulic acid were 38.5-166.7 ?g/mL(r=0.999 6),15.9~254.5 ?g/mL(r=0.999 9),22.1-353 ?g/mL(r =0.999 3),6.30-201.5 ?g/mL(r=0.999 9),respectively.The average recoveries were 97.57%,97.40%,98.86%,96.37%,respectively.The RSD were 2.1%,1.1%,0.70%,1.3%,respectively.

CONCLUSION:

The method is rapid,simple and accurate,and can be used for quality control of Chaihu Shugan Powder.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional Patent Medicine Año: 1992 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Traditional Patent Medicine Año: 1992 Tipo del documento: Artículo