SRAP Analysis of genetic diversity about germplasm in Salvia miltiorrhiza from different sources / 中草药

ABSTRACT

Objective To solve the key problem on classification and identification of Salvia miltiorrhiza,which was urgently needed to be solved in breeding of S. miltiorrhiza. Methods Sequence-related amplified polymorphism (SRAP) was carried out to analyze the genetic variation of 48 germplasm in S. miltiorrhiza from different sources. Results The data showd that 120 alleles had been found using 15 pairs of primer which had been selected from 100 pairs. Unweighted pair-group method with arithmetical averages (UPGMA) cluster analysis was used to classify the germplasm of S. miltiorrhiza. Two groups could be divided and three subgroups were contained in every group. Conclusion A complex relationship exists between the genetic distance and space distance of differernt S. miltiorrhiza. The genetic diversity in different geographical populations of S. miltiorrhiza in China is rich. The results show that the genetic distance of different germplasm will increase with the stage of domestication.