Determination of Chlorogenic Acid in Shuanghuanglian Keli by HPLC / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-580795
ABSTRACT
Objective To establish the method for detemining the content of chlorogenic acid in Shuanghuanglian Keli by HPLC.Methods Using Diamonsil ODS1 C18 column,with methanol-water-glacial acetic acid(15851) as the mobile phase,detection wavelength as 324 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.060~1.210 ?g for chlorogenic acid and the equation was Y=105 427X+586.43,r2=0.999 5.The average recovery was 99.3% and RSD was 0.82%(n=6).Conclusion This method was simple,accurate and proper,and the reduplication of the result was good,which provide scientific quantitative analysis method of chlorogenic acid in Shuanghuanglian Keli.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Año:
2006
Tipo del documento:
Artículo
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