Content Determination of Total Coumarins in Fructus Cnidii / 中国中医药信息杂志

ABSTRACT

Objective To establish the method for content determination of total coumarins in Fructus Cnidii.Methods The content of the total coumarins was determined by UV spectrophotometry.Results The optimal detective wavelength was 320 nm for the total coumarins.Satisfactroy linear relationship with the absorbability was achieved when the mass concentration of osthole was within the range of 2～12 ?g/mL(r=0.999 5).The average recovery rate was 99.39%(RSD=2.67%,n=6).Conclusion The method is simple,accurate and sensitive,and it can be used for the quality control of Fructus cnidii and its preparation.