Effects of dressing change with insulin on the expression of HIF-1? and VEGF in deep lI burn wound healing in diabetic rats / 重庆医科大学学报

ABSTRACT

Objective:To study the effect of dressing with insulin on the expression of HIF-1?and VEGF in deepⅡ degree burn wound healing of diabetic rats and explore the possible mechanisms. Methods:42 rats,either male or female,were established models of deepⅡ burn wounds with diabetes,which was induced by streptozotocin (STZ). They were completely randomly divided into two groups,that is the insulin group and the control group. Dressing changes with insulin and saline dressings twice daily,respectively. Full-thickness tissues of the wounds were collected respectively in the 1st,2nd and 3rd weeks after the treatment. The expression of hypoxia inducible factor-1?mRNA (HIF-1?mRNA)was detected with RT-PCR;and the positive staining of HIF-1?and VEGF and microvessel density in the wound healing were determined with IHC. Results:Compared with the control,the healing rate was obviously increased, the expression of HIF-1?mRNA and the positive expression of HIF-1?were significantly decreased (P