Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo
The Korean Journal of Parasitology
;
: 233-240, 2001.
Artículo
en Inglés
| WPRIM
| ID: wpr-58168
ABSTRACT
Although some reports have been published on the protective effect of antibodies to Toxoplasma gondii surface membrane proteins, few address the inhibitory activity of antibodies to dense granular proteins (GRA proteins). Therefore, we performed a series of experiments to evaluate the inhibitory effects of monoclonal antibodies (mAbs) to GRA proteins (GRA2, 28 kDa; GRA6, 32 kDa) and surface membrane protein (SAG1, 30 kDa) on the invasion of T. gondii tachyzoites. Passive immunization of mice with one of three mAbs following challenge with a lethal dose of tachyzoites significantly increased survival compared with results for mice treated with control ascites. The survival times of mice challenged with tachyzoites pretreated with anti-GRA6 or anti-SAG1 mAb were significantly increased. Mice that received tachyzoites pretreated with both mAb and complement had longer survival times than those that received tachyzoites pretreated with mAb alone. Invasion of tachyzoites into fibroblasts and macrophages was significantly inhibited in the anti-GRA2, anti-GRA6 or anti-SAG1 mAb pretreated group. Pretreatment with mAb and complement inhibited invasion of tachyzoites in both fibroblasts and macrophages. These results suggest that specific antibodies to dense-granule molecules may be useful for controlling infection with T. gondii.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Toxoplasma
/
Proteínas Protozoarias
/
Toxoplasmosis
/
Inmunización Pasiva
/
Fibroblastos
/
Interacciones Huésped-Parásitos
/
Macrófagos
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
/
Antígenos de Protozoos
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2001
Tipo del documento:
Artículo
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