TC-1 Stromal Cells Enhance Retro viral-Mediated Gene Transfer into Murine Marrow Hematopoietic Cells / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)1996.
Article
en Zh
| WPRIM
| ID: wpr-581918
Biblioteca responsable:
WPRO
ABSTRACT
Objective: To investigate the optimal gene transfer protocols of hematopoietic cells mediated by retrovirus. Methods: Murine bone marrow cells were infected by co-culture with murine bone marrow stromal cell line TC-1 or retro-virus packaging cells or retrovirus supernatant. Human mdr-1 and enhanced green fluorescent protein (EGFP) were used as report genes. Results: Stromal cells could greatly increase the gene transfer efficiency when compared with that of supernatant transfection. Transduction efficiency was highest when infected BM cells were co-cultured with virus producer cells. Conculsion: It may be clinically feasible in gene therapy to perform retroviral transduction by co-culture of target cells with stromal cells or cell lines.
Texto completo:
1
Índice:
WPRIM
Tipo de estudio:
Guideline
Idioma:
Zh
Revista:
Chinese Journal of Cancer Biotherapy
Año:
1996
Tipo del documento:
Article